DOI: http://dx.doi.org/10.18203/2349-3259.ijct20162798

Bioclinical stress of Rogor pesticide in the fish Amphipnous cuchia

Brijendra Pratap Mishra, Mohan Lal Kanojia, Sajjan Lal Verma, Jhansi Lakshmi Lingidi

Abstract


Background: The impact of acute and sub lethal toxicity of synthetic Rogor on some biochemical and hematological parameters of a fish Amphipnous cuchia was estimated aquatic environment by various pollutants like pesticides, detergents, discharge of effluents and heavy metals induce changes in the biochemical, hematological and behavioral aspects of inhabitants. These pollutants cause serious effects on growth, biochemistry, physiology, neurochemistry and survival rate of the aquatic organism.

Methods: The present research work is one such attempt to investigate the effect of organophosphorous pesticide Rogor on biochemical and hematological parameters of fish Amphipnous cuchia. The main objective of this study was to investigate the level of AST, ALT, hematocrit and TLC in fish Amphipnous cuchia exposed 24 to 96 hours to four different concentrations of Rogor pesticide.

Results: Rogor toxicity resulted in a significant maximum increase (89.15%) in level of aspartate amino transferase (7.87±0.14/18.31±0.09) and alanine amino transferase (76.65%, 5.28±0.11/3.03±0.14) enzyme. Regarding to hematological parameter, significant maximum increase (77.12%) in total leukocyte count (27,400±298/15,470±286) and maximum decrease (66.07%) in hematocrit (6.20±0.04 /18.31±0.09) level was observed.  

Conclusions: In this study it is concluded that exposure to sublethal/lethal concentration of Rogor results in a significant alterations in different biochemical and hematological parameters and this kind of biochemical and physiological changes may directly affect the survivability of these fishes in these natural habitat.


Keywords


Rogor pesticide, Pesticide toxicity, Aspartate transferase, Alanine transferase, TLC, Hematocrit

Full Text:

PDF

References


Siddiqui MKJ, Rahman MF, Mahboob M, Anjum F, Mustafa M. Species differences in brain acetylcholinesterase and neuropathic target esterase response to monocrotophos. J Environ Sci Health B. 1988;23:291-9.

Qadri YH, Swamy AN, Rao JV. Species differences in brain acetylcholinesterase response to monocrotophos in vitro. Ecotoxicol Environ Saf. 1994;28:91-8.

Gupta PK. Pesticide exposure-Indian scene. Toxicology. 2004;198:83-90.

Tomlin CDS. The Pesticide Manual, 12th Edition, Farnham, Surrey, UK: British Crop Protection Council; 2000.

Farm Chemicals International, November 1999, WOW! 2000.

ACP. Evaluation of Dimethoate, Evaluation of Fully approved products no.86, Pesticide Safety Directorate, Ministry of Agriculture, Fisheries and Food, London, 1993.

Fisher SW. Changes in the toxicity of three pesticides as a function of environmental pH and temperature. Bull Environ Contam Toxicol. 1991;46:197-202.

Richmonds CR, Dutta HM. Effect of Malathion on the brain acetylcholinesterase activity of bluegill sunfish Lepomis macrochirus. Bull Environ Contam Toxicol. 1992;49:431-5.

Dutta HM, Arends DA. Effects of endosulfan on brain acetylcholinesterase activity in juvenile bluegill sunfish. Environ Res. 2003;91:157-62.

Soreq H, Zakut H. Human Acetylcholinesterases and Anticholinesterases. Academic Press, San Diego; 1993.

Dutta HM, Munshi HSD, Dutta GR, Singh NK, Adhikari S, Richmonds CR. Age related differences in the inhibition of brain acetylcholinesterase activity of Heteropneustes fossillis (Bloch) by Malathion. Comp Biochem Physiol C. 1995;11:331-4.

Nehez M, Desi I. The effects of Dimethoate on Bone marrow cell chromosomes of Rats in subchronic four generation experiments. Ecotoxicology and environmental safety. 1996;33:103-9.

Siddiqui MKJ, Rahman MF, Mustafa M, Bhalerao UT. A comparative study of blood changes and brain acetylcholinesterase inhibition by monocrotophs and its analogues in rats. Ecotoxicol Environ Saf. 1991;21:283-9.

Tripathi AK, Upadhyay S, Mantu Bhuiyan, Bhattacharya PR. A review on prospects of essential oils as biopesticide in insect – pest management. Journal of Pharmacology and Physiology. 2009;15:52-63.

Rajyasree M, Neeraja P. Aspartate and alanine aminotransferase activities in fish tissue subcellular fractionation on exposure to ambient urea. Indian J Fish. 1989;36:88-91.

Oluah NS. Plasma aspartate aminotransferase activity in the catfish Clarias albopunctatus exposed to sublethal zinc and mercurys. Bull Environ Contam Toxicol. 1999;63:343-9.

Pandey BN, Chancjal AK, Singh MP. Effect of Malathion on oxygen consumption and blood of Channa Punctatus (Bloch). Indian J Zool. 1987;27:95-100.

Sethi N, Singh RK. Serum inorganic phosphorous levels of fresh water mud-eel, Amphinous Cuchia under intoxication of pesticide sumithion. Ad Biosci. 1987;2:175-8.

Tandon RS, Sethi N, Singh RK, Dayal R, Nath D. Toxic effects of pesticide sumithion in blood urea levels of sdof mud-eel, Amphinous Cuchia. Ind J Bio Res. 1986;2:166-8.

Darbal R, Chaturvedi LD. Folidol induced changes in same haematological values of Heteropneustes Fossils at low temperature. J Adv Zool. 1983;4:92-6.

Koundinya RP, Ramamurthi RP, Ramamurthi R. Haematological studies in Sarothredon mossambica (Peters) exposed to lethal (LC50/40 hours) concentration of sumithion and sevin. Curr Sci. 1979;48:877.